《植物生理学报》 2012, 48(12): 1187-1192

外源蔗糖和Ca²⁺对荞麦幼苗耐盐性的影响

陈晓云¹, 刘洪庆¹, 李发良², 苏丽萍², 杨洪兵^1,*

1青岛农业大学生命科学学院, 山东省高校植物生物技术重点实验室, 山东青岛266109; 2四川省凉山州农业科学研究所高山 作物研究站, 四川凉山616150

通信作者：杨洪兵；E-mail: hbyang@qau.edu.cn；Tel: 0532-88030393

摘　要：

以盐敏感荞麦品种‘TQ-0808’为实验材料, 在100 mmol•L-1 NaCl胁迫下研究添加不同浓度蔗糖和Ca2+处理对荞麦幼苗 耐盐性的影响。不同浓度的外源蔗糖和Ca2+处理均能明显降低盐胁迫下荞麦叶片的质膜透性和丙二醛(MDA)含量, 明显增 加荞麦叶片的超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)活性及净光合速率, 且外源Ca2+处理的增加效果明显好 于外源蔗糖处理。说明外源蔗糖和Ca2+处理能明显改善盐胁迫下荞麦幼苗的耐盐性, 对盐胁迫具有较好的缓解作用, 外源 蔗糖和Ca2+处理的最适浓度分别为6 mmol•L-1和20 mmol•L-1。

关键词：荞麦; 盐胁迫; 蔗糖; Ca2+; 耐盐性

收稿：2012-11-05   修定：2012-12-10

资助：山东省自然科学基金(ZR2010CL019)。

Effects of Exogenous Sucrose and Ca²⁺ on Salt Tolerance of Buckwheat Seedlings

CHEN Xiao-Yun¹, LIU Hong-Qing¹, LI Fa-Liang², SU Li-Ping², YANG Hong-Bing^1,*

¹Key Laboratory of Plant Biotechnology in Universities of Shandong, College of Life Sciences, Qingdao Agricultural University, Qingdao, Shandong 266109, China; ²Research Station of Alpine Crop, Liangshan Institute of Agricultural Science, Liangshan, Sichuan 616150, China

Corresponding author: YANG Hong-Bing; E-mail: hbyang@qau.edu.cn; Tel: 0532-88030393

Abstract:

Salt-sensitive buckwheat (Fagopyrum esculentum) variety ‘TQ-0808’ was used as experimental material. Under the stress of 100 mmol·L^-1 NaCl, the effects of different concentrations of sucrose and Ca²⁺treatment on salt tolerance of buckwheat seedlings were studied. The results showed that different concentrations of exogenous sucrose and Ca²⁺ treatment could significantly reduce the plasmalemma permeability and malondialdehyde (MDA) content of buckwheat leaves under salt stress, and significantly increase superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities and net photosynthetic rate of buckwheat leaves, and the increase effect of exogenous Ca²⁺ treatment was more obviously better than that of exogenous sucrose. These indicated that exogenous sucrose and Ca²⁺ treatment could significantly improve salt tolerance of buckwheat seedlings and had a better remission to the salt stress, and the optimal concentrations of exogenous sucrose and Ca²⁺ treatment were 6 mmol·L^-1 and 20 mmol·L^-1 respectively.

Key words: buckwheat (Fagopyrum esculentum Moench); salt stress; sucrose; Ca²⁺; salt tolerance